DRAFT OECD GUIDELINE FOR THE TESTING OF CHEMICALS Rodent alkaline single cell gel electrophoresis (Comet) assay INTRODUCTION
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چکیده
1. OECD Test Guidelines (TGs) are available for a wide range of in vitro genotoxicity assays that are able to detect DNA damage, gene mutation and/or chromosomal aberrations. There are TGs for in vivo endpoints (i.e. chromosomal aberrations, gene mutations and DNA repair as unscheduled DNA synthesis); however, these do not directly measure DNA damage. The alkaline single cell gel electrophoresis (Comet) assay fulfills the need for practical and widely available in vivo tests for measurement of DNA damage induction in multiple tissues.
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Oecd Guideline for the Testing of Chemicals in Vivo Mammalian Alkaline Comet Assay Introduction
1. The in vivo alkaline comet (single cell gel electrophoresis) assay (hereafter called simply the comet assay) is used for the detection of DNA strand breaks in cells or nuclei isolated from multiple tissues of animals, usually rodents, that have been exposed to potentially genotoxic material(s). The comet assay has been reviewed and recommendations have been published by various expert groups...
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